Genotyping and primer details


Genotyping and primer details for SALK_054203 - At3g02500
Line ID SALK_054203
Locus At3g02500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:519638 of line SALK_054203 Chr3:519638
   
Insertion allele amplicon LBb3 - 73xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 73xo: 5'-ACTTTAGTGCATACTCAACAGGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:520575
   
Insertion allele amplicon LBb3 - CG63
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG63: 5'-ATAACCTTTCGAGAAAACAATGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:519011
   
wt amplicon (over InsPos) CG64 - CG63
wt amplicon start Chr3:519011
wt amplicon end Chr3:519903
wt amplicon size 893 bp
Locus specific primer CG63: 5'-ATAACCTTTCGAGAAAACAATGGA-3'
2nd locus specific primer CG64: 5'-TGTTGATGCAAATTTCAAGAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11