Genotyping and primer details


Genotyping and primer details for SALK_054291 - At1g78920
Line ID SALK_054291
Locus At1g78920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29673810 of line SALK_054291 Chr1:29673810
   
Insertion allele amplicon LBb3 - 52dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52dl: 5'-TTTTCTCGGTATGAGTCAGGAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29674366
   
wt amplicon (over InsPos) 03ej - 52dl
wt amplicon start Chr1:29673350
wt amplicon end Chr1:29674366
wt amplicon size 1017 bp
Locus specific primer 52dl: 5'-TTTTCTCGGTATGAGTCAGGAACT-3'
2nd locus specific primer 03ej: 5'-ACACGTTTCCAAATACTAAGTGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11