Genotyping and primer details


Genotyping and primer details for SALK_054409c - At1g03457
Line ID SALK_054409c
Locus At1g03457 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:863142 of line SALK_054409c Chr1:863142
   
Insertion allele amplicon LBb3 - 22ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22ty: 5'-ACTCGCCATCTGCATACTTAACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:862765
   
wt amplicon (over InsPos) 23uu - 22ty
wt amplicon start Chr1:862765
wt amplicon end Chr1:863718
wt amplicon size 954 bp
Locus specific primer 22ty: 5'-ACTCGCCATCTGCATACTTAACTT-3'
2nd locus specific primer 23uu: 5'-GAAAACCCTAATTTTCTCCGTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11