Sequences from confirmation amplicon


Confirmation sequences for line SALK_054409c and hit At1g03457 are shown.
Line ID SALK_054409c
Locus At1g03457 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:863142
Primers used for PCR 22ty - LBb3, show primer details
   
Sequence Name 34-K117180-SALK_054409C-22ty
GenBank Accession LN485207 [GenBank]
Sequencing primer gene specific primer, 22ty: 5'-ACTCGCCATCTGCATACTTAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117180-SALK_054409C-22ty
GAAGTGAATTCTATAATTCGAGAGAGGGATAAGAGTGTACATAGCCCATAAGCATCCTAA
TGTTCAGATACCGGCCACAGAAAAATCGAAAGAAGCAAGGATATATATATACACCAAATT
TTTGTTAAAGATCGCTCAACAAAAAGTCAACACAATGAAGCAAAAGTTTTCTCTTGGTAT
TTGGGTTCCTATAATCTCATTCCTAGAGATGTTCTAGCTTTTTGCATCTTGCACGTTTTA
GTGACCACTGACATACATCTATAGTGGACCAATGTGGTTTCTTGGGTTTGAAGAAATCAG
CTAGAGAGGATTTGAAACGAACTGATAGGACAAATCTGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAAAT
   
Sequence Name 13-K117179-SALK_054409C-R210
GenBank Accession LN484651 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117179-SALK_054409C-R210
TTCGTTTCAATCCTCTCTAGCTGATTTCTTCAAACCCAAGAAACCACATTGGTCCACTAT
AGATGTATGTCAGTGGTCACTAAAACGTGCAAGATGCAAAAAGCTAGAACATCTCTAGGA
ATGAGATTATAGGAACCCAAATACCAAGAGAAAACTTTTGCTTCATTGTGTTGACTTTTT
GTTGAGCGATCTTTAACAAAAATTTGGTGTATATATATATCCTTGCTTCTTTCGATTTTT
CTGTGGCCGGTATCTGAACATTAGGATGCTTATGGGCTATGTACACTCTTATCCCTCTCT
CGAATTATAGAATTCACTTTCTTATGATATGTTTCAGGCATCTAGTCCGTTGCAAGTTAA
GTATGCGAATGGCGAGT

Last Updated on 06.12.2018 16:11