Genotyping and primer details


Genotyping and primer details for SALK_054774c - At5g65920
Line ID SALK_054774c
Locus At5g65920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26364692 of line SALK_054774c Chr5:26364692
   
Insertion allele amplicon LBb3 - 23ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23ty: 5'-TAGAATTCAACACATCGAAACTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26364191
   
wt amplicon (over InsPos) 25vb - 23ty
wt amplicon start Chr5:26364191
wt amplicon end Chr5:26365108
wt amplicon size 918 bp
Locus specific primer 23ty: 5'-TAGAATTCAACACATCGAAACTCG-3'
2nd locus specific primer 25vb: 5'-GAAGGTGGAGTCTTTGTGATCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11