Genotyping and primer details


Genotyping and primer details for SALK_055351c - At5g01890
Line ID SALK_055351c
Locus At5g01890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:342412 of line SALK_055351c Chr5:342412
   
Insertion allele amplicon LBb3 - 09cn
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09cn: 5'-TAGTTTAATCACCGGTATTGCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:341763
   
wt amplicon (over InsPos) 17dn - 09cn
wt amplicon start Chr5:341763
wt amplicon end Chr5:342591
wt amplicon size 829 bp
Locus specific primer 09cn: 5'-TAGTTTAATCACCGGTATTGCCTC-3'
2nd locus specific primer 17dn: 5'-GCCAAGTAAAGATCAAGAGTTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11