Genotyping and primer details


Genotyping and primer details for SALK_055373c - At3g50060
Line ID SALK_055373c
Locus At3g50060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18559137 of line SALK_055373c Chr3:18559137
   
Insertion allele amplicon LBb3 - 27ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27ea: 5'-TTACTCCACAATCCCTAAAACCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18558166
   
wt amplicon (over InsPos) 02ft - 27ea
wt amplicon start Chr3:18558166
wt amplicon end Chr3:18559303
wt amplicon size 1138 bp
Locus specific primer 27ea: 5'-TTACTCCACAATCCCTAAAACCAC-3'
2nd locus specific primer 02ft: 5'-ACTAATAAACAGACGCACCGAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11