Genotyping and primer details


Genotyping and primer details for SALK_055490c - At3g18440
Line ID SALK_055490c
Locus At3g18440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6329756 of line SALK_055490c Chr3:6329756
   
Insertion allele amplicon LBb3 - 32mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32mh: 5'-TCTCTCGGTTTCTGCTTATAGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6329243
   
wt amplicon (over InsPos) 10ng - 32mh
wt amplicon start Chr3:6329243
wt amplicon end Chr3:6329944
wt amplicon size 702 bp
Locus specific primer 32mh: 5'-TCTCTCGGTTTCTGCTTATAGCTC-3'
2nd locus specific primer 10ng: 5'-TGAAATGTTCCCAGATTAAGCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11