Sequences from confirmation amplicon


Confirmation sequences for line SALK_055490c and hit At3g18440 are shown.
Line ID SALK_055490c
Locus At3g18440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6329756
Primers used for PCR 32mh - LBb3, show primer details
   
Sequence Name 85-K114279-SALK_055490C-32mh
GenBank Accession LN486287 [GenBank]
Sequencing primer gene specific primer, 32mh: 5'-TCTCTCGGTTTCTGCTTATAGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K114279-SALK_055490C-32mh
CCTATATGGGCTGGAGAGGATCTTCATAACCTTGTCGTGAAGAATTTTATGAATGTTGCG
ACTTCCCTTGAAGGTTAGTATAACAAAGGTTTTGGCTTTTCTGAGTCTCGGGAAGGAGAT
GCTTTACTGAGGATTGTCTTTGGTCTTATAGGTTGTGTGAATGGATATCTCCGCTGTCTT
GAATACGAGAGAATCCCATCCAAAATCCTCACGTACCAAGCTTCAGAGGATCCTGTTTAC
AAGGGTTATAGATCAGCTGTTGAGTCAACCAGCCAAGAAGAGTCTCTGGTATTTGTCATT
GGGGATCATTTTCGATAAGTCGGTTTTTATTCCATTGCTTCTCATCTCTTAATTAGTTTG
TGTTTTCTCTGGTGTAATTGCAAAGATGAGTTTCGCAATATGGGAGCCACCACATGGACC
ATACAAGTCATTTAACTACCCATGGAAGATGGTGTAAACAAATTGACGCTTAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGATGGTTCCG
   
Sequence Name 71-K114278-SALK_055490C-R210
GenBank Accession LN486036 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114278-SALK_055490C-R210
CATCTTCCTGGGTAGTTAAATGACTTGTATGGTCCATGTGGTGGCTCCCATATTGCGAAA
CTCATCTTTGCAATTACACCAGAGAAAACACAAACTAATTAAGAGATGAGAAGCAATGGA
ATAAAAACCGACTTATCGAAAATGATCCCCAATGACAAATACCAGAGACTCTTCTTGGCT
GGTTGACTCAACAGCTGATCTATAACCCTTGTAAACAGGATCCTCTGAAGCTTGGTACGT
GAGGATTTTGGATGGGATTCTCTCGTATTCAAGACAGCGGAGATATCCATTCACACAACC
TATAAGACCAAAGACAATCCTCAGTAAAGCATCTCCTTCCCGAGACTCAGAAAAGCCAAA
ACCTTTGTTATACTAACCTTCAAGGGAAGTCGCAACATTCATAAAATTCTTCACGACAAG
GTTATGAAGATCCTCTCCAGCCCATATAGGATAGATAAACATATTAACACCTAAACTAAC
ACCAGCACCAAGAGCTATNAGCAGAACCCCGAGAG

Last Updated on 06.12.2018 16:11