Genotyping and primer details


Genotyping and primer details for SALK_055713c - At3g16920
Line ID SALK_055713c
Locus At3g16920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5777418 of line SALK_055713c Chr3:5777418
   
Insertion allele amplicon LBb3 - 87ns
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 87ns: 5'-AAGAAACTTCAAAACCAAGCAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5778027
   
wt amplicon (over InsPos) 15or - 87ns
wt amplicon start Chr3:5777044
wt amplicon end Chr3:5778027
wt amplicon size 984 bp
Locus specific primer 87ns: 5'-AAGAAACTTCAAAACCAAGCAAAA-3'
2nd locus specific primer 15or: 5'-CAGTAGATCCACTTTCAAAGCCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11