Genotyping and primer details


Genotyping and primer details for SALK_055721c - At1g50960
Line ID SALK_055721c
Locus At1g50960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18889566 of line SALK_055721c Chr1:18889566
   
Insertion allele amplicon LBb3 - 45wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45wm: 5'-TGAAACCTCTGAAAGAATGATGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18889977
   
wt amplicon (over InsPos) 21xa - 45wm
wt amplicon start Chr1:18888992
wt amplicon end Chr1:18889977
wt amplicon size 986 bp
Locus specific primer 45wm: 5'-TGAAACCTCTGAAAGAATGATGTG-3'
2nd locus specific primer 21xa: 5'-TTTCCAAGTTAGATATTCGTTGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11