Genotyping and primer details


Genotyping and primer details for SALK_055969c - At5g48680
Line ID SALK_055969c
Locus At5g48680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19745030 of line SALK_055969c Chr5:19745030
   
Insertion allele amplicon LBb3 - 31ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31ux: 5'-TCCAGTCACAAGTTTGTTTTCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19744462
   
wt amplicon (over InsPos) 63vl - 31ux
wt amplicon start Chr5:19744462
wt amplicon end Chr5:19745572
wt amplicon size 1111 bp
Locus specific primer 31ux: 5'-TCCAGTCACAAGTTTGTTTTCTTG-3'
2nd locus specific primer 63vl: 5'-CTGTTTGGTTGTTGACTCTGTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11