Genotyping and primer details


Genotyping and primer details for SALK_056441c - At5g19060
Line ID SALK_056441c
Locus At5g19060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6373922 of line SALK_056441c Chr5:6373922
   
Insertion allele amplicon LBb3 - 08tw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 08tw: 5'-ATCTCACCGTTGTTTTACTCTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6373567
   
wt amplicon (over InsPos) 08ue - 08tw
wt amplicon start Chr5:6373567
wt amplicon end Chr5:6374273
wt amplicon size 707 bp
Locus specific primer 08tw: 5'-ATCTCACCGTTGTTTTACTCTTCC-3'
2nd locus specific primer 08ue: 5'-AAGGTTAGACCAGTGAATACCGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11