Genotyping and primer details

Genotyping and primer details for SALK_056908c - At1g20950
Line ID SALK_056908c
Locus At1g20950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7299738 of line SALK_056908c Chr1:7299738
Insertion allele amplicon LBb3 - 24ty
Locus specific primer 24ty: 5'-TTGCACAGATTTGAAGTTAGATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7300239
wt amplicon (over InsPos) 21uu - 24ty
wt amplicon start Chr1:7299224
wt amplicon end Chr1:7300239
wt amplicon size 1016 bp
Locus specific primer 24ty: 5'-TTGCACAGATTTGAAGTTAGATGG-3'
2nd locus specific primer 21uu: 5'-ACCTTCTACTGCTCTTGCTTGAAC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11