Sequences from confirmation amplicon


Confirmation sequences for line SALK_056908c and hit At1g20950 are shown.
Line ID SALK_056908c
Locus At1g20950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7299738
Primers used for PCR 24ty - LBb3, show primer details
   
Sequence Name 36-K117180-SALK_056908C-24ty
GenBank Accession LN485240 [GenBank]
Sequencing primer gene specific primer, 24ty: 5'-TTGCACAGATTTGAAGTTAGATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117180-SALK_056908C-24ty
TCATTGGCAAATTTTTGTCTTTAATTTTTACTAAACCAAGAATATATATTNGTGGTTTTT
GAAGGCGTAATATCAAACACAGATGCAGCTCATCTCGCTGAATTTTTTGCTGCTGCAAAA
TGCTCAACTAAGGTCCTAATTTCTTTTTCTACAGTTTCATTTTAAATTCCTTTATAAAAA
TGCTACGGTAATTAAGCTCCTATGAAATTACAGGTGGTTGGTGTTCCAGTTACTACAAAT
GGAGATCTCAAAAATCAGTTTGTGGAGGCAAACGTGGGTTTTGACACCATATGCAAGGTA
AAATGTAAACTTTAACCATCTGGTTACTTTGTCTTAGTTATCTTTTGTAGCTATTGCAGC
AACATGATGCCGTTGTCCCTCGTAAGTCTTTGCAGAAACTATATGTTATTGTTTGCTAAT
GATATTTTGCGGCATACAGGTGAACTCATAACACATTGACGCTTAGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 15-K117179-SALK_056908C-R210
GenBank Accession LN484705 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117179-SALK_056908C-R210
CTGTATGCCGCAAATATCATTAGCAAACAATAACNATATAGTTTCTGCAAAGACTTACGA
GGGACAACGGCATCATGTTGCTGCAATAGCTACAAAAGATAACTAAGACAAAGTAACCAG
ATGGTTAAAGTTTACATTTTACCTTGCATATGGTGTCAAAACCCACGTTTGCCTCCACAA
ACTGATTTTTGAGATCTCCATTTGTAGTAACTGGAACACCAACCACCTGTAATTTCATAG
GAGCTTAATTACCGTAGCATTTTTATAAAGGAATTTAAAATGAAACTGTAGAAAAAGAAA
TTAGGACCTTAGTTGAGCATTTTGCAGCAGCAAAAAATTCAGCGAGATGAGCTGCATCTG
TGTTTGATATTACGCCTTCAAAAACCACAATATATATTCTTGGTTTAGTAAAAATTAAAG
ACAAAAATTTGCCAATGAATATAAGTAAAAACGCTAGGTACCTCCAATGATAACAAGGCC
ATCTAACTTCAATCCTGTGCAA

Last Updated on 06.12.2018 16:11