Genotyping and primer details


Genotyping and primer details for SALK_057421c - At5g05760
Line ID SALK_057421c
Locus At5g05760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1729153 of line SALK_057421c Chr5:1729153
   
Insertion allele amplicon LBb3 - 55kk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55kk: 5'-ATCCAGGTCAGAGAGAGACTGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1728677
   
wt amplicon (over InsPos) 13lz - 55kk
wt amplicon start Chr5:1728677
wt amplicon end Chr5:1729616
wt amplicon size 940 bp
Locus specific primer 55kk: 5'-ATCCAGGTCAGAGAGAGACTGAAG-3'
2nd locus specific primer 13lz: 5'-TTATGCTTGAAGGTCAAAATGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11