Genotyping and primer details


Genotyping and primer details for SALK_057795c - At4g09420
Line ID SALK_057795c
Locus At4g09420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5962734 of line SALK_057795c Chr4:5962734
   
Insertion allele amplicon LBb3 - 12di
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12di: 5'-AGATCAATCTGAAAGCAACCATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5963160
   
wt amplicon (over InsPos) 17sl - 12di
wt amplicon start Chr4:5962157
wt amplicon end Chr4:5963160
wt amplicon size 1004 bp
Locus specific primer 12di: 5'-AGATCAATCTGAAAGCAACCATCT-3'
2nd locus specific primer 17sl: 5'-AGCCTTGAGATGGAGACAATAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11