Genotyping and primer details

Genotyping and primer details for SALK_057925c - At4g25890
Line ID SALK_057925c
Locus At4g25890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13160478 of line SALK_057925c Chr4:13160478
Insertion allele amplicon LBb3 - 56ew
Locus specific primer 56ew: 5'-AATTACCTACGCCACAAGAAATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13159783
wt amplicon (over InsPos) 41gc - 56ew
wt amplicon start Chr4:13159783
wt amplicon end Chr4:13160726
wt amplicon size 944 bp
Locus specific primer 56ew: 5'-AATTACCTACGCCACAAGAAATTG-3'
2nd locus specific primer 41gc: 5'-TCATGTGTTAGGAAATGACTCTCG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11