Sequences from confirmation amplicon


Confirmation sequences for line SALK_057925c and hit At4g25890 are shown.
Line ID SALK_057925c
Locus At4g25890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13160478
Primers used for PCR 56ew - LBb3, show primer details
   
Sequence Name 63-K111283-SALK_057925C-56ew
GenBank Accession LN485819 [GenBank]
Sequencing primer gene specific primer, 56ew: 5'-AATTACCTACGCCACAAGAAATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K111283-SALK_057925C-56ew
TGCAAACAGGATTATACACAAATATTGATTTGTACTAGTTTCCGGAGCCATGTAAACTAA
AGTCACCTACTAAAACAGAGAAGGCAACCAAAAACAAATTGAGTGATTTAGCTATTACAA
AGCTGTAACCTTTAGGTATCATTGTTAACCTATAAAACAAGCTACATTGACGCAAAATCA
CATTTGATGCAATGATAAGAGAACTATAGTGAGCCATGACACTTGTATCACTGATCAACG
ACCATAATTACTGTGAATGAATCATTCATCTGAAAATGCAAGCTCTGATTCTCACACAGT
ATTGCCAATTTCAAAGATGCACGATCTCAAACTGTAAGCTTGTTCGAGCAAGAGAGACTT
ATACCTGGAATACGGCGGAGGTAGGGGAGACAAGAGAAAACGAAGACTGAACGCCACCGG
AGGAATCAGCGGAGAGAGATACCTGAACGAGCTTACGCTGGAGCTCGTAGGTAGAAGAAG
CTGAAGATTCAAGTTCTCCTTCGTGTTGCTTCGCGCTCCACGCGCCGCCTCCATTCTTGC
ATACGAATGTGAATACTCCCATCTTCTTCAATGTCTGCTAAAATCGATGGCGATAGTGAG
AGATTTCTGGAAAAAGTGACGATCGAGAGTGGTTTTGTTTTTATATATTGTGGTGTAAAC
AAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGT
TTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGA
GAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT
GG
   
Sequence Name 47-K111282-SALK_057925C-R210
GenBank Accession LN485435 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K111282-SALK_057925C-R210
CATTTGTTACCCACAATATATAAAACAAAACCACTCTCGATCGTCACTTTTTCCAGAAAT
CTCTCACTATCGCCATCGATTTTAGCAGACATTGAAGAAGATGGGAGTATTCACATTCGT
ATGCAAGAATGGAGGCGGCGCGTGGAGCGCGAAGCAACACGAAGGAGAACTTGAATCTTC
AGCTTCTTCTACCTACGAGCTCCAGCGTAAGCTCGTTCAGGTATCTCTCTCCGCTGATTC
CTCCGGTGGCGTTCAGTCTTCGTTTTCTCTTGTCTCCCCTACCTCCGCCGTATTCCAGGT
ATAAGTCTCTCTTGCTCGAACAAGCTTACAGTTTGAGATCGTGCATCTTTGAAATTGGCA
ATACTGTGTGAGAATCAGAGCTTGCATTTTCAGATGAATGATTCATTCACAGTAATTATG
GTCGTTGATCAGTGATACAAGTGTCATGGCTCACTATAGTTCTCTTATCATTGCATCAAA
TGTGATTTTGCGTCAATGTAGCTTGTTTTATAGGTTAACAATGATACCTAAAGGTTACAG
CTTTGTAATAGCTAAATCACTCAATTTGTTTTTGGTTGCCTTCTCTGTTTTAGTAGGTGA
CTTTAGTTTACATGGCTCCGGAAACTAGTACAAATCAATATTTGTGTATAATTCCTGTTT
TGCATTGTAATTTTTATCAATTGGAATCAATTTCTTGTGG

Last Updated on 06.12.2018 16:11