Genotyping and primer details


Genotyping and primer details for SALK_057940c - At2g41310
Line ID SALK_057940c
Locus At2g41310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17223284 of line SALK_057940c Chr2:17223284
   
Insertion allele amplicon LBb3 - 12vb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12vb: 5'-AAACGAAGAAAGCTAATCTAGGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17224084
   
wt amplicon (over InsPos) 04vj - 12vb
wt amplicon start Chr2:17222796
wt amplicon end Chr2:17224084
wt amplicon size 1289 bp
Locus specific primer 12vb: 5'-AAACGAAGAAAGCTAATCTAGGGG-3'
2nd locus specific primer 04vj: 5'-TGTCTTTTCCATCTCTGGCTAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11