Genotyping and primer details


Genotyping and primer details for SALK_058455 - At1g23050
Line ID SALK_058455
Locus At1g23050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8169099 of line SALK_058455 Chr1:8169099
   
Insertion allele amplicon R204 - BN01
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BN01: 5'-CCATTCGTTAACCTCCATACTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8169438
   
Insertion allele amplicon LBb3 - BN02
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BN02: 5'-TTAGTCATATCCGACACACCATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8168703
   
wt amplicon (over InsPos) BN02 - BN01
wt amplicon start Chr1:8168703
wt amplicon end Chr1:8169438
wt amplicon size 736 bp
Locus specific primer BN01: 5'-CCATTCGTTAACCTCCATACTGTT-3'
2nd locus specific primer BN02: 5'-TTAGTCATATCCGACACACCATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11