Genotyping and primer details


Genotyping and primer details for SALK_058509 - At5g06700
Line ID SALK_058509
Locus At5g06700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2063623 of line SALK_058509 Chr5:2063623
   
Insertion allele amplicon LBb3 - 19jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19jd: 5'-GTTGTTTGGTTTCTATTCACGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2064236
   
wt amplicon (over InsPos) 02kp - 19jd
wt amplicon start Chr5:2063137
wt amplicon end Chr5:2064236
wt amplicon size 1100 bp
Locus specific primer 19jd: 5'-GTTGTTTGGTTTCTATTCACGGTT-3'
2nd locus specific primer 02kp: 5'-TTTTATTTCAACCGCATTATTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11