Genotyping and primer details


Genotyping and primer details for SALK_059264c - At3g22170
Line ID SALK_059264c
Locus At3g22170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7823587 of line SALK_059264c Chr3:7823587
   
Insertion allele amplicon R204 - SC01
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC01: 5'-CACCAACCTACATGACTGATGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7824096
   
wt amplicon (over InsPos) 28ah - SC01
wt amplicon start Chr3:7823229
wt amplicon end Chr3:7824096
wt amplicon size 868 bp
Locus specific primer SC01: 5'-CACCAACCTACATGACTGATGTTC-3'
2nd locus specific primer 28ah: 5'-CTGCACAGAGAGATCGTAAACAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11