Sequences from confirmation amplicon


Confirmation sequences for line SALK_059264c and hit At3g22170 are shown.
Line ID SALK_059264c
Locus At3g22170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7823587
Primers used for PCR SC01 - R204, show primer details
   
Sequence Name 56-K106282-SALK_059264C-R210
GenBank Accession LN485634 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K106282-SALK_059264C-R210
CGTCATTTGTAAGGATGTAAGGATTTCATATGTACTGCGAGGGGATGGAAGAAAGATGAC
AACACTGAAGAACATTAAGAGTGTGTCTGCAGAGATACCCTTTATACTCAAATAACCGAC
AGATGCAAGAGACTTCAGCCTTTGTTTGGTTCCATGTTACCATGAAGTCTTGGTTGTTTT
CAAAATCCTGCACTCTAAAAGTAGAACATGTGGCATCTCGGTTCTCCTCCCTAGGACTAC
ACGCAATCGCACCCAAAACTTCAATCTGGAACTTTTTGAAAACTGCAGGAGTATAGACTT
CAGAAACACTCTTCTCAAATGGCGATGGAGACTTCATTGCTGGCTGCTTGTTCCACATTT
CAGAATCAGCTTTGGCTTCTTCTTCACATCTATCTTGCAAAACTGTATCATATACCTTTA
CAAATTCTTGTACACTAGTCTTCTTATGCATATACTTGTCGAAGAAAGCATTAATACTAT
CAGCTCGTTGAGATGTAGACATCCCAGCCAAAAGAACATCAGTCATGTAGGTTGGTG
   
Sequence Name 72-K106283-SALK_059264C-SC01
GenBank Accession LN486047 [GenBank]
Sequencing primer gene specific primer, SC01: 5'-CACCAACCTACATGACTGATGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K106283-SALK_059264C-SC01
CGAGCTGATAGTATTAATGCTTTCTTCGACAGTATATGCATAAGAAGACTAGTGTACAAG
AATTTGTAAAGGTATATGATACAGTTTTGCAAGATAGATGTGAAGAAGAAGCCAAAGCTG
ATTCTGAAATGTGGAACAAGCAGCCAGCAATGAAGTCTCCATCGCCATTTGAGAAGAGTG
TTTCTGAAGTCTATACTCCTGCAGTTTTCAAAAAGTTCCAGATTGAAGTTTTGGGTGCGA
TTGCGTGTAGTCCTAGGGAGGAGAACCGAGATGCCACATGTTCTACTTTTAGAGTGCAGG
ATTTTGAAAACAACCAAGACTTCATGGTAACATGGAACCAAACAAAGGCTGAAGTCTCTT
GCATCTGTCGGTTATTTGAGTATAAAGGGTATCTCTGCAGACACACTCTTAATGTTCTTC
AGTGTTGTCATCTTTCTTCCATCCCCTCGCAGTACATATTGAAATCCTTACATCCTTACA
AATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTT
TTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAG
AGTTGCAGCAAGGGNTCCACG

Last Updated on 06.12.2018 16:11