Genotyping and primer details


Genotyping and primer details for SALK_059288 - At5g39760
Line ID SALK_059288
Locus At5g39760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15912268 of line SALK_059288 Chr5:15912268
   
Insertion allele amplicon LBb3 - 32ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32ux: 5'-TGATCCAGATAACAACAACGACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15911860
   
wt amplicon (over InsPos) 62vl - 32ux
wt amplicon start Chr5:15911860
wt amplicon end Chr5:15912729
wt amplicon size 870 bp
Locus specific primer 32ux: 5'-TGATCCAGATAACAACAACGACTC-3'
2nd locus specific primer 62vl: 5'-GTATTATTCCTCATCGAGCGAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11