Sequences from confirmation amplicon


Confirmation sequences for line SALK_059288 and hit At5g39760 are shown.
Line ID SALK_059288
Locus At5g39760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15912268
Primers used for PCR 32ux - LBb3, show primer details
   
Sequence Name 25-K117668-SALK_059288C-R210
GenBank Accession LN485014 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117668-SALK_059288C-R210
CATTTGTTTACCCACAATATATCCTGCATTGTGTAACACATTGCAGTTTGTCCTTTGACG
CAATGTGTTATTCCGATCTGCCGGCAAAAATCACGAACATCGTCTTCGTCACGTTTTTGC
ATCTTCCAACCAACACGTTCGGCGAATTCATGCATCTTCTCTTTCTGAAACTGGCTGAAT
TTTGTTCGGAATCTTTTACGAGATCCGTGAAGAGTATGTTGGTGGTGGTGGTGGTGGTTG
TTTCCGGCGGAGAAGTTAAGATCGGAGAAAGAAGCTAAGTTGTTGTTGTTGTTATTAGTA
CCGGAGAGAGATAAGAGCATGTAAGAGGAAGAGATTGGTGGAGGAGAAGCTGAGTTAGGA
CTACGTGGTGGTGGAGGAGGTGGTGGTGGTGCAGGACACTAAGAG
   
Sequence Name 62-K117669-SALK_059288C-32ux
GenBank Accession LN485802 [GenBank]
Sequencing primer gene specific primer, 32ux: 5'-TGATCCAGATAACAACAACGACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117669-SALK_059288C-32ux
CCTCACCACCNTCATCATCCACCACCACCACCACCTCCTCCACCACCACGTAGTCCTAAC
TCAGCTTCTCCTCCACCAATCTCTTCCTCTTACATGCTCTTATCTCTCTCCGGTACTAAT
AACAACAACAACAACTTAGCTTCTTTCTCCGATCTTAACTTCTCCGCCGGAAACAACCAC
CACCACCACCACCAACATACTCTTCACGGATCTCGTAAAAGATTCCGAACAAAATTCAGC
CAGTTTCAGAAAGAGAAGATGCATGAATTCGCCGAACGTGTTGGTTGGAAGATGCAAAAA
CGTGACGAAGACGATGTTCGTGATTTTTGCCGGCAGATCGGAATAACACATTGCGTCAAA
GGACAAACTGCAATGTGTTACACAATGCAGGATATATTGTGGTGTAAACAAATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAAT
CGGCAAAATAAAAAAA

Last Updated on 06.12.2018 16:11