Genotyping and primer details


Genotyping and primer details for SALK_059682 - At4g29100
Line ID SALK_059682
Locus At4g29100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14344017 of line SALK_059682 Chr4:14344017
   
Insertion allele amplicon LBb3 - 46wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46wm: 5'-CTTTCTTAGGCTCTTCACTTGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14344462
   
wt amplicon (over InsPos) 50xb - 46wm
wt amplicon start Chr4:14343333
wt amplicon end Chr4:14344462
wt amplicon size 1130 bp
Locus specific primer 46wm: 5'-CTTTCTTAGGCTCTTCACTTGCAT-3'
2nd locus specific primer 50xb: 5'-GATTCCTTCAGAGTCAAATTGAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11