Genotyping and primer details


Genotyping and primer details for SALK_059686c - At1g66180
Line ID SALK_059686c
Locus At1g66180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24647497 of line SALK_059686c Chr1:24647497
   
Insertion allele amplicon LBb3 - 22dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22dh: 5'-CGGAAATGTTAAGCTTCTTCAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24648073
   
wt amplicon (over InsPos) 07dz - 22dh
wt amplicon start Chr1:24647192
wt amplicon end Chr1:24648073
wt amplicon size 882 bp
Locus specific primer 22dh: 5'-CGGAAATGTTAAGCTTCTTCAAAC-3'
2nd locus specific primer 07dz: 5'-TCTCTCTCACTTCCACAAAACTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11