Genotyping and primer details


Genotyping and primer details for SALK_062093c - At1g34340
Line ID SALK_062093c
Locus At1g34340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12531817 of line SALK_062093c Chr1:12531817
   
Insertion allele amplicon LBb3 - 03rk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03rk: 5'-TCCATCTCCATACGATCTTCTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12530962
   
wt amplicon (over InsPos) 21sl - 03rk
wt amplicon start Chr1:12530962
wt amplicon end Chr1:12532133
wt amplicon size 1172 bp
Locus specific primer 03rk: 5'-TCCATCTCCATACGATCTTCTCTT-3'
2nd locus specific primer 21sl: 5'-CATGAAAAACAAAGCAAATCTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11