Genotyping and primer details


Genotyping and primer details for SALK_062789 - At1g15700
Line ID SALK_062789
Locus At1g15700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5402936 of line SALK_062789 Chr1:5402936
   
Insertion allele amplicon LBb3 - 28ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28ea: 5'-AGACATTGATTTTCCTCTGAGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5403424
   
wt amplicon (over InsPos) 06fg - 28ea
wt amplicon start Chr1:5402464
wt amplicon end Chr1:5403424
wt amplicon size 961 bp
Locus specific primer 28ea: 5'-AGACATTGATTTTCCTCTGAGCAT-3'
2nd locus specific primer 06fg: 5'-CCTAAATTGGTTGTCTTCTTCGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11