Genotyping and primer details


Genotyping and primer details for SALK_062853c - At2g33270
Line ID SALK_062853c
Locus At2g33270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14104957 of line SALK_062853c Chr2:14104957
   
Insertion allele amplicon LBb3 - 58us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 58us: 5'-TATTGAATCACACAAGCAAATGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14105247
   
wt amplicon (over InsPos) 38vl - 58us
wt amplicon start Chr2:14104560
wt amplicon end Chr2:14105247
wt amplicon size 688 bp
Locus specific primer 58us: 5'-TATTGAATCACACAAGCAAATGGT-3'
2nd locus specific primer 38vl: 5'-TGTTCATTCCATTAACAACTCGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11