Genotyping and primer details


Genotyping and primer details for SALK_063265c - At4g16600
Line ID SALK_063265c
Locus At4g16600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9351231 of line SALK_063265c Chr4:9351231
   
Insertion allele amplicon LBb3 - 20ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20ht: 5'-CAGTAATAAGATGCGTCCATTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9351742
   
wt amplicon (over InsPos) 16ix - 20ht
wt amplicon start Chr4:9350683
wt amplicon end Chr4:9351742
wt amplicon size 1060 bp
Locus specific primer 20ht: 5'-CAGTAATAAGATGCGTCCATTTGA-3'
2nd locus specific primer 16ix: 5'-TTGAGAATGTCCAGAACAAGACAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11