Genotyping and primer details


Genotyping and primer details for SALK_063337 - At3g56710
Line ID SALK_063337
Locus At3g56710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21007056 of line SALK_063337 Chr3:21007056
   
Insertion allele amplicon LBb3 - 57qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 57qz: 5'-AAACCCATCAAAGTTCGTTACATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21007397
   
wt amplicon (over InsPos) 06rz - 57qz
wt amplicon start Chr3:21006733
wt amplicon end Chr3:21007397
wt amplicon size 665 bp
Locus specific primer 57qz: 5'-AAACCCATCAAAGTTCGTTACATC-3'
2nd locus specific primer 06rz: 5'-ACGGGTACATTGGGTATATGAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11