Genotyping and primer details


Genotyping and primer details for SALK_063470c - At2g35930
Line ID SALK_063470c
Locus At2g35930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15083559 of line SALK_063470c Chr2:15083559
   
Insertion allele amplicon LBb3 - 18gg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18gg: 5'-AAATGAAAGTGACAATAGCCTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15082839
   
wt amplicon (over InsPos) 03hv - 18gg
wt amplicon start Chr2:15082839
wt amplicon end Chr2:15083663
wt amplicon size 825 bp
Locus specific primer 18gg: 5'-AAATGAAAGTGACAATAGCCTGGT-3'
2nd locus specific primer 03hv: 5'-GTATGACTCTCAAGCCAGAGGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11