Genotyping and primer details


Genotyping and primer details for SALK_064044 - At5g53635
Line ID SALK_064044
Locus At5g53635 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21787221 of line SALK_064044 Chr5:21787221
   
Insertion allele amplicon LBb3 - 40wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40wu: 5'-TATCTACGCCTCGAAATTGTTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21787572
   
wt amplicon (over InsPos) 17xf - 40wu
wt amplicon start Chr5:21786397
wt amplicon end Chr5:21787572
wt amplicon size 1176 bp
Locus specific primer 40wu: 5'-TATCTACGCCTCGAAATTGTTTCT-3'
2nd locus specific primer 17xf: 5'-TATACAAAGAGTAACATGCAGGCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11