Genotyping and primer details


Genotyping and primer details for SALK_064247c - At3g29180
Line ID SALK_064247c
Locus At3g29180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:11151109 of line SALK_064247c Chr3:11151109
   
Insertion allele amplicon LBb3 - 42cp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42cp: 5'-TTTGTGGACTAACTTTTGCTTGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:11150388
   
wt amplicon (over InsPos) 09dz - 42cp
wt amplicon start Chr3:11150388
wt amplicon end Chr3:11151497
wt amplicon size 1110 bp
Locus specific primer 42cp: 5'-TTTGTGGACTAACTTTTGCTTGTG-3'
2nd locus specific primer 09dz: 5'-AATGGCAAACTCAAGAAGATGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11