Sequences from confirmation amplicon


Confirmation sequences for line SALK_064247c and hit At3g29180 are shown.
Line ID SALK_064247c
Locus At3g29180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11151109
Primers used for PCR 42cp - LBb3, show primer details
   
Sequence Name 14-K109155-SALK_064247C-42cp
GenBank Accession LN484662 [GenBank]
Sequencing primer gene specific primer, 42cp: 5'-TTTGTGGACTAACTTTTGCTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K109155-SALK_064247C-42cp
ACTTTAGCGTTGATTCATTATGTGTAATGATCTTGACCTTTGTACCTCACTGGCAGTCCC
TAAATATCCTGCTGCAATGTTCCTTGGGGACAGTGATGGACAAGGCATGAGCATTGTACT
TTATTTTAAATTACGAGACAATCACGAAAAAGAAACCTCTCAGCAATATCAGGAGAGCAT
CAAGGTAGCTTGAAAAGCCATTCCTTTATGACATTCATCACATCCCATGACTGATCTAAA
TGCTTACCGAGTTCTTTTTGTTTTCGGTTTCAAAACAGAAACTTGTCAATGATGAGATGG
AGAAGGTAAAAGGGTTTGCTAAAGACAGCAACGTTGCTTTCCGTGAAAGGCTTAAGATAG
TAGCTGGACTTGTTAACCCCGAAGATCTAGCTCTAAGCTCGACTGAAAAGAAGCTTGTAC
AGGCTTACAATGAAAAACCTGTCCTCTCTCGTCCTCAACACAACTTCTTTAAGGTTTGAG
CACACCATCACTACCTCTTAAACACACAACAGAAGTCACCTAATTAAAATTTACTTTTAC
CTCTCTCCACAGGGTCCAAACTACTTCGAGATTGATCTGGATGTACATCGATTCAGCTAC
ATATCTCGAAAAGGACTTGAAGCATTCAGAGATCGACTAAAAAACGGAACTCTTGATCTC
GGCTTAACCATCCAGGTAACTTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TTATGTACTGGGGTGGTTTTTCTTTT
   
Sequence Name 06-K109154-SALK_064247C-R210
GenBank Accession LN484423 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K109154-SALK_064247C-R210
GTTCCTGGATGGTTAGCCGAGATCAGAGTTCCGTTTTTTAGTCGATCTCTGAATGCTTCA
AGTCCTTTTCGAGATATGTAGCTGAATCGATGTACATCCAGATCAATCTCGAAGTAGTTT
GGACCCTGTGGAGAGAGGTAAAAGTAAATTTTAATTAGGTGACTTCTGTTGTGTGTTTAA
GAGGTAGTGATGGTGTGCTCAAACCTTAAAGAAGTTGTGTTGAGGACGAGAGAGGACAGG
TTTTTCATTGTAAGCCTGTACAAGCTTCTTTTCAGTCGAGCTTAGAGCTAGATCTTCGGG
GTTAACAAGTCCAGCTACTATCTTAAGCCTTTCACGGAAAGCAACGTTGCTGTCTTTAGC
AAACCCTTTTACCTTCTCCATCTCATCATTGACAAGTTTCTGTTTTGAAACCGAAAACAA
AAAGAACTCGGTAAGCATTTAGATCAGTCATGGGATGTGATGAATGTCATAAAGGAATGG
CTTTTCAAGCTACCTTGATGCTCTCCTGATATTGCTGAGAGGTTTCTTTTTCGTGATTGT
CTCGTAATTTAAAATAAAGTACAATGCTCATGCCTTCTCCATCACTGTCCCCAAGGAACA
TTGCAGCAGGATAGGTTGGTAACTGCCAGTGAGGTACAAAGGTCAAGATCATTTACACAT
ATATGTATATTCAACGCTAATAAAGCTTCTGATGAAAAACACAAGCCAAAGTT

Last Updated on 06.12.2018 16:11