Genotyping and primer details


Genotyping and primer details for SALK_064292 - At5g43380
Line ID SALK_064292
Locus At5g43380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17419862 of line SALK_064292 Chr5:17419862
   
Insertion allele amplicon LBb3 - 36uv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36uv: 5'-CATCAGCTAAAACTTTGTGTCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17419360
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11