Genotyping and primer details


Genotyping and primer details for SALK_064531c - At3g26400
Line ID SALK_064531c
Locus At3g26400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9667835 of line SALK_064531c Chr3:9667835
   
Insertion allele amplicon LBb3 - 85by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 85by: 5'-AATCAGTCTAGGGTTTCGGATTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9667078
   
wt amplicon (over InsPos) 31dk - 85by
wt amplicon start Chr3:9667078
wt amplicon end Chr3:9667975
wt amplicon size 898 bp
Locus specific primer 85by: 5'-AATCAGTCTAGGGTTTCGGATTTT-3'
2nd locus specific primer 31dk: 5'-TAAAGATTGACTCCTGTGGCATAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11