Genotyping and primer details


Genotyping and primer details for SALK_065296c - At1g61730
Line ID SALK_065296c
Locus At1g61730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22794679 of line SALK_065296c Chr1:22794679
   
Insertion allele amplicon LBb3 - 71sa
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 71sa: 5'-TCCTTTAGGTCCCCAAATAAACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22793834
   
wt amplicon (over InsPos) 05sx - 71sa
wt amplicon start Chr1:22793834
wt amplicon end Chr1:22795272
wt amplicon size 1439 bp
Locus specific primer 71sa: 5'-TCCTTTAGGTCCCCAAATAAACTT-3'
2nd locus specific primer 05sx: 5'-ATTGTATCTGGGTCAAAATGGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11