Sequences from confirmation amplicon


Confirmation sequences for line SALK_065296c and hit At1g61730 are shown.
Line ID SALK_065296c
Locus At1g61730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22794679
Primers used for PCR 71sa - LBb3, show primer details
   
Sequence Name 48-K116392-SALK_065296C-71sa
GenBank Accession LN485461 [GenBank]
Sequencing primer gene specific primer, 71sa: 5'-TCCTTTAGGTCCCCAAATAAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116392-SALK_065296C-71sa
AGCTTTACAAACTAGGTTCGTTCCTTCCTTCTTTACCAATATACTTCTTCCTCATACTCC
TAATCTTATCCATGAATTGATTCTTACTAACCTCAAAGCTAATCGATTTCTTCAAGAAAT
CGTAAAACGCATTAGTATCTACATAAGGAGACTTCCCTGTATCAGCTTTGAAATCAATCA
TTCCTTGTAACACTAAGATTTCGTCTTCTTCACTCCATAGTCTTTGAAAACCTCCTGGTT
TCTTCACACTCTCTTCATCTTTCTTCACTCGTTTCGTTGAAGTCGTAGCAGCAGCTTCAC
TCGCTGGCCGTTTTGTTCCTGATTTCATCGCCGGTAAAGCTAAAGTAGCTGACGACGACG
TTGGATCCTCTTTCTTCTTTAGATTCACTGTGTTTAAAGCGATTGTCTTCCCAGATCCAG
AATTCGGTGGATTTGTTGATTCAGAATCCGAATCTGTTTCAGTCTCGGATCCAGAATCTG
AATCAGCGGCGGTGGAGACGGCGGTGGATTTAGCTGGAGGAGCGGCGGCGGTTGTGGCGG
AAGGGGATTTGATTCGAATTTTGATTGGTACGTCTTCTTCAGATGAAGAGGAATCGTCTG
AAGCTTCACCAGCTTCGGAAGTCTCGACGTCATCTTCGTCGCTTGAAGTTGCCGTTGGTG
GATCTTCCAGTGGATTGAGTTTCTTCGTCATTGGAAATTTTCAAGGAGGCAAATCGGAAT
CCTTCAAGCAGAAGAGAAGAGAGAAGCTTTTTAGGGTTTGTGAGGAGAGGACGGCAAAAG
TGTTTGGGAATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGNGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGG
   
Sequence Name 23-K116391-SALK_065296C-R210
GenBank Accession LN484954 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K116391-SALK_065296C-R210
TTTGTTACCCACAATATTCCCAACACTTTTGCCGTCCTCTCCTCACAAACCCTAAAAAGC
TTCTCTCTTCTCTTCTGCTTGAAGGATTCCGATTTGCCTCCTTGAAAATTTCCAATGACG
AAGAAACTCAATCCACTGGAAGATCCACCAACGGCAACTTCAAGCGACGAAGATGACGTC
GAGACTTCCGAAGCTGGTGAAGCTTCAGACGATTCCTCTTCATCTGAAGAAGACGTACCA
ATCAAAATTCGAATCAAATCCCCTTCCGCCACAACCGCCGCCGCTCCTCCAGCTAAATCC
ACCGCCGTCTCCACCGCCGCTGATTCAGATTCTGGATCCGAGACTGAAACAGATTCGGAT
TCTGAATCAACAAATCCACCGAATTCTGGATCTGGGAAGACAATCGCTTTAAACACAGTG
AATCTAAAGAAGAAAGAGGATCCAACGTCGTCGTCAGCTACTTTAGCTTTACCGGCGATG
AAATCAGGAACAAAACGGCCAGCGAGTGAAGCTGCTGCTACGACTTCAACGAAACGAGTG
AAGAAAGATGAAGAGAGTGTGAAGAAACCAGGAGGTTTTCAAAGACTATGGAGTGAAGAA
GACGAAATCTTAGTGTTACAAGGAATGATTGATTTCAAAGCTGATACAGGGAAGTCTCCT
TATGTAGATACTAATGCGTTTTACGATTTCTTGAAGAAATCGATTAGCTTTGAGGTTAGT
AAGAATCAATTCATGGATAAGATTAGGAGTTTAAGGAAGAAGTATATTGGTAAAGAAGGA
AGGAACGAACCTAGTTTTGTGAAAGCTCATGATAAGAAAGCTTTTGAATTGTCTAAGTTT
ATTTG

Last Updated on 06.12.2018 16:11