Genotyping and primer details


Genotyping and primer details for SALK_065342 - At5g27430
Line ID SALK_065342
Locus At5g27430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9688493 of line SALK_065342 Chr5:9688493
   
Insertion allele amplicon LBb3 - 15ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15ii: 5'-GGATTGTTCTGGAAGTTTGTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9687741
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11