Genotyping and primer details


Genotyping and primer details for SALK_065859c - At4g39960
Line ID SALK_065859c
Locus At4g39960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18535070 of line SALK_065859c Chr4:18535070
   
Insertion allele amplicon LBb3 - 69kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 69kh: 5'-GTGTGTTCAATTTCCAGGATTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18534621
   
wt amplicon (over InsPos) 12lz - 69kh
wt amplicon start Chr4:18534621
wt amplicon end Chr4:18535556
wt amplicon size 936 bp
Locus specific primer 69kh: 5'-GTGTGTTCAATTTCCAGGATTTCT-3'
2nd locus specific primer 12lz: 5'-TTGAACCTCTACTACCCATTCCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11