Genotyping and primer details


Genotyping and primer details for SALK_066347c - At5g64560
Line ID SALK_066347c
Locus At5g64560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25807194 of line SALK_066347c Chr5:25807194
   
Insertion allele amplicon LBb3 - 44kq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44kq: 5'-CACGTCTTGATATCTTTACAGGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25807718
   
wt amplicon (over InsPos) 11lz - 44kq
wt amplicon start Chr5:25806737
wt amplicon end Chr5:25807718
wt amplicon size 982 bp
Locus specific primer 44kq: 5'-CACGTCTTGATATCTTTACAGGCA-3'
2nd locus specific primer 11lz: 5'-GGCTTCAACCGATAATTATTGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11