Genotyping and primer details


Genotyping and primer details for SALK_066908c - At5g15410
Line ID SALK_066908c
Locus At5g15410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5005735 of line SALK_066908c Chr5:5005735
   
Insertion allele amplicon LBb3 - 17ey
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17ey: 5'-CGATGAAAACAGTAACCTCCAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5006308
   
wt amplicon (over InsPos) 04go - 17ey
wt amplicon start Chr5:5005407
wt amplicon end Chr5:5006308
wt amplicon size 902 bp
Locus specific primer 17ey: 5'-CGATGAAAACAGTAACCTCCAAAT-3'
2nd locus specific primer 04go: 5'-AGTGATAAATCTTGGGGAGGAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11