Genotyping and primer details


Genotyping and primer details for SALK_066923c - At2g20030
Line ID SALK_066923c
Locus At2g20030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8648945 of line SALK_066923c Chr2:8648945
   
Insertion allele amplicon LBb3 - 34ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34ux: 5'-CTTCATCTGATTTGACGTTTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8648609
   
wt amplicon (over InsPos) 61vl - 34ux
wt amplicon start Chr2:8648609
wt amplicon end Chr2:8649543
wt amplicon size 935 bp
Locus specific primer 34ux: 5'-CTTCATCTGATTTGACGTTTTTGA-3'
2nd locus specific primer 61vl: 5'-TTTCTGATGGGCTCTGACTTTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11