Genotyping and primer details


Genotyping and primer details for SALK_066961 - At5g67230
Line ID SALK_066961
Locus At5g67230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26823623 of line SALK_066961 Chr5:26823623
   
Insertion allele amplicon LBb3 - CG39
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG39: 5'-GACTTATGTACGGACTTTTCAGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26822982
   
wt amplicon (over InsPos) CG40 - CG39
wt amplicon start Chr5:26822982
wt amplicon end Chr5:26823745
wt amplicon size 764 bp
Locus specific primer CG39: 5'-GACTTATGTACGGACTTTTCAGGC-3'
2nd locus specific primer CG40: 5'-ATCATCCAACAAGCTGAGATCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11