Sequences from confirmation amplicon


Confirmation sequences for line SALK_066961 and hit At5g67230 are shown.
Line ID SALK_066961
Locus At5g67230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26823623
Primers used for PCR CG39 - LBb3, show primer details
   
Sequence Name 30-K109819-SALK_066961C-CG39
GenBank Accession LN485125 [GenBank]
Sequencing primer gene specific primer, CG39: 5'-GACTTATGTACGGACTTTTCAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K109819-SALK_066961C-CG39
ACGATTTGGTTTGGATCGTTGTGGAAGCTGGTGGAATCACTAACGAGACTGCTTCGTTTA
TCGCAAAATCAGGATTAAAGACGATTCACTTAGGATTCGATCAGAAAATGCCTAATACAT
GGGAAGATCGTCACAAATTGGAGACCAAAATGAGACTTCACGCCTTGAGGTTAAATCTTT
GATTCACATTTTGTTTTGCTGTTTTGATTGATTGATTCTTATTTGACTTGAAATTTATTG
TTCTTGTGTTGATGTTTGCTCATCAGAGTTGTGAGAGAGAAGAAGTTAGATGGGATTGTT
ATGTTTGCTGATGATAGCAATATGCATAGTATGGAGCTTTTTGATGAGATTCAAACTGTG
AAATGGTTTGGTGCTCTATCTGTTGGTATACTTGCTCATTCTGGTAATGCAGATGAATTA
TCATCGATCTTGAAGAATGAACAAGGGAAGAACAAAGAGAAACCTTCAATGCCAATCCAA
GGTCCTAGTTGTAATTCCTCTGAGAAATTAGTGGGTTGGCACATTTTCAACACACAGCCT
TATGCCAAGAAGACTGCAGTGTATATCGATGAGACAAATAAACCACCATATTTACGCTTA
GACCACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACC
AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
   
Sequence Name 14-K109818-SALK_066961C-R210
GenBank Accession LN484663 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K109818-SALK_066961C-R210
GGTGGTTATTTGTCTCATCGATATACACTGCAGTCTTCTTGGCATAAGGCTGTGTGTTGA
AAATGTGCCAACCCACTAATTTCTCAGAGGAATTACAACTAGGACCTTGGATTGGCATTG
AAGGTTTCTCTTTGTTCTTCCCTTGTTCATTCTTCAAGATCGATGATAATTCATCTGCAT
TACCAGAATGAGCAAGTATACCAACAGATAGAGCACCAAACCATTTCACAGTTTGAATCT
CATCAAAAAGCTCCATACTATGCATATTGCTATCATCAGCAAACATAACAATCCCATCTA
ACTTCTTCTCTCTCACAACTCTGATGAGCAAACATCAACACAAGAACAATAAATTTCAAG
TCAAATAAGAATCAATCAATCAAAACAGCAAAACAAAATGTGAATCAAAGATTTAACCTC
AAGGCGTGAAGTCTCATTTTGGTCTCCAATTTGTGACGATCTTCCCATGTATTAGGCATT
TTCTGATCGAATCCTAAGTGAATCGTCTTTAATCCTGATTTTGCGATAAACGAAGCAGTC
TCGTTAGTGATTCCACCAGCTTCCACAACGATCCAAACCAAATCGTACGGAACAAGCATA
AGCGAGTGCATAACTCCGGTCAAATGAAGCGCCTGAAAAGTCCGTACATAAG

Last Updated on 06.12.2018 16:11