Genotyping and primer details


Genotyping and primer details for SALK_067094c - At3g21310
Line ID SALK_067094c
Locus At3g21310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7498129 of line SALK_067094c Chr3:7498129
   
Insertion allele amplicon LBb3 - 26dj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26dj: 5'-TCGTTTAGACTTACTTCTGGTCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7498620
   
wt amplicon (over InsPos) 10dz - 26dj
wt amplicon start Chr3:7497895
wt amplicon end Chr3:7498620
wt amplicon size 726 bp
Locus specific primer 26dj: 5'-TCGTTTAGACTTACTTCTGGTCCC-3'
2nd locus specific primer 10dz: 5'-TGGTTCTTGGAATCTCAGTCATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11