Sequences from confirmation amplicon


Confirmation sequences for line SALK_067094c and hit At3g21310 are shown.
Line ID SALK_067094c
Locus At3g21310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7498129
Primers used for PCR 26dj - LBb3, show primer details
   
Sequence Name 58-K109345-SALK_067094C-26dj
GenBank Accession LN485684 [GenBank]
Sequencing primer gene specific primer, 26dj: 5'-TCGTTTAGACTTACTTCTGGTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K109345-SALK_067094C-26dj
AGGCCCGTCCTCGTCACAGAACCCATGAAACTATATCTTGATCTAGAGACATAACGGTAG
ACAAAGTTGAAACCGCGAAGAGGAATACACGCTTCAGATAGTAAAACAAACCATTCGTTA
GAGATATCGAGCAACGCGTTAGCCAAAAGCCTCCTTTCAGCATCACACATACTCATCTCT
CCCCAAGCTACATGCTACACACAAATAAAGAAACTCCGAAGTGAGATAAACCAAATCAAG
ACACAAGAAGCAACATTAATACATTGATCAACAAAAGTACCTGACTTGGGATCTGTCTTC
TGTAAAACACAGATGAGCTTGGGAAATCTGATCTATAATTTGGCAAAGTATGAACATAGA
TTGAGTAAAAGCCCTCATGCCCTTTGAAAAACCTTTCCCAAAGTGGAGCAAATGGCAAAG
GTCCTTTGGTGAGAAACAAATTGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 50-K109344-SALK_067094C-R210
GenBank Accession LN485501 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K109344-SALK_067094C-R210
CGTCATTTGTTACACCACAATATATCCAATTTGTTTCTCACCAAAGGACCTTTGCCATTT
GCTCCACTTTGGGAAAGGTTTTTCAAAGGGCATGAGGGCTTTTACTCAATCTATGTTCAT
ACTTTGCCAAATTATAGATCAGATTTCCCAAGCTCATCTGTGTTTTACAGAAGACAGATC
CCAAGTCAGGTACTTTTGTTGATCAATGTATTAATGTTGCTTCTTGTGTCTTGATTTGGT
TTATCTCACTTCGGAGTTTCTTTATTTGTGTGTAGCATGTAGCTTGGGGAGAGATGAGTA
TGTGTGATGCTGAAAGGAGGCTTTTGGCTAACGCGTTGCTCGATATCTCTAACGAATGGT
TTGTTTTACTATCTGAAGCGTGTATTCCTCTTCGCGGTTTCAACTTTGTCTACCGTTATG
TCTCTAGATCAAGATATAGTTTCATGGGTTCTGTTGACGAGGACGGGCCTTACGGGAGAG
GCAGATATAGCTATGCAATGGGACCAGAAGTAAGTCTAAACG

Last Updated on 06.12.2018 16:11